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Simple and large-scale chromosomal engineering of mouse zygotes via in vitro and in vivo electroporation
The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has facilitated dramatic progress in the field of genome engineering. Whilst microinjection of the Cas9 protein and a single guide RNA (sgRNA) into mouse zygotes is a widespread method for producing genetically engine...
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| 出版年: | Sci Rep |
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| 主要な著者: | , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Nature Publishing Group UK
2019
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6789149/ https://ncbi.nlm.nih.gov/pubmed/31604975 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-50900-y |
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