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Efficient mouse genome engineering by CRISPR-EZ (CRISPR RNP Electroporation of Zygotes) technology
CRISPR/Cas9 technology has transformed mouse genome editing with unprecedented precision, efficiency, and ease; however, the current practice of microinjecting CRISPR reagents into pronuclear-stage embryos remains rate-limiting. We thus developed CRISRP-EZ (CRISPR RNP Electroporation of Zygotes), an...
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| Publicado no: | Nat Protoc |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6296855/ https://ncbi.nlm.nih.gov/pubmed/29748649 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2018.012 |
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