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Simple knockout by electroporation of engineered endonucleases into intact rat embryos

Engineered endonucleases, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system, provide a powerful approach for genome editing in animals. However, the...

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Autores principales: Kaneko, Takehito, Sakuma, Tetsushi, Yamamoto, Takashi, Mashimo, Tomoji
Formato: Artigo
Lenguaje:Inglês
Publicado: Nature Publishing Group 2014
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4180828/
https://ncbi.nlm.nih.gov/pubmed/25269785
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep06382
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