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Squaramate‐Modified Nucleotides and DNA for Specific Cross‐Linking with Lysine‐Containing Peptides and Proteins

Squaramate‐linked 2′‐deoxycytidine 5′‐O‐triphosphate was synthesized and found to be good substrate for KOD XL DNA polymerase in primer extension or PCR synthesis of modified DNA. The resulting squaramate‐linked DNA reacts with primary amines to form a stable diamide linkage. This reaction was used...

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Publicat a:Angew Chem Int Ed Engl
Autors principals: Ivancová, Ivana, Pohl, Radek, Hubálek, Martin, Hocek, Michal
Format: Artigo
Idioma:Inglês
Publicat: John Wiley and Sons Inc. 2019
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6771961/
https://ncbi.nlm.nih.gov/pubmed/31328344
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201906737
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