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利用CRISPR/Cas9技术构建敲除G6PD基因c.392G>T (p.131G>V)突变位点的HEK293T稳定细胞株
OBJECTIVE: To establish a stable HEK293T cell line with c.392G>T (p.131G>V) mutation site knockout in G6PD gene using CRISPR/Cas9 technique. METHODS: We designed 4 pairs of small guide RNA (sgRNA) for G6PD c.392G>T(p.131G>V) mutation site, and constructed exogenous PX458 plasmids express...
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| Publicat a: | Nan Fang Yi Ke Da Xue Xue Bao |
|---|---|
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
南方医科大学学报编辑部
2019
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6765671/ https://ncbi.nlm.nih.gov/pubmed/31068316 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.12122/j.issn.1673-4254.2019.03.10 |
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