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利用框内缺失法构建变异链球菌srtA基因缺失株
OBJECTIVE: To construct srtA-gene deletion mutant of Streptococcus mutans (S. mutans) UA159 with IFDC2 cassette through overlapping polymerase chain reaction (PCR) and allelic homologous recombination. METHODS: First, the upstream and downstream fragments surrounding the srtA and IFDC2 cassette were...
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| Publicado no: | Hua Xi Kou Qiang Yi Xue Za Zhi |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
华西口腔医学杂志编辑部
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7041158/ https://ncbi.nlm.nih.gov/pubmed/29333770 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7518/hxkq.2017.06.005 |
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