利用CRISPR/Cas9系统构建稳定敲除4.1R基因的RAW264.7细胞株

OBJECTIVE: To construct a cell model of 4.1R gene knockout in murine macrophage cell line RAW264.7 using CRISPR/ Cas9 technique. METHODS: Three high-grade small-guide RNAs (sgRNAs) that could specifically identify 4.1R gene were synthesized and inserted into lentiCRISPRv2 plasmid. RAW264.7 cells wer...

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Bibliografiske detaljer
Udgivet i:Nan Fang Yi Ke Da Xue Xue Bao
Format: Artigo
Sprog:Inglês
Udgivet: 南方医科大学学报编辑部 2017
Fag:
基础研究
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6744011/
https://ncbi.nlm.nih.gov/pubmed/29292253
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3969/j.issn.1673-4254.2017.12.08
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