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利用CRISPR/Cas9系统构建稳定敲除4.1R基因的RAW264.7细胞株
OBJECTIVE: To construct a cell model of 4.1R gene knockout in murine macrophage cell line RAW264.7 using CRISPR/ Cas9 technique. METHODS: Three high-grade small-guide RNAs (sgRNAs) that could specifically identify 4.1R gene were synthesized and inserted into lentiCRISPRv2 plasmid. RAW264.7 cells wer...
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| Izdano u: | Nan Fang Yi Ke Da Xue Xue Bao |
|---|---|
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
南方医科大学学报编辑部
2017
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6744011/ https://ncbi.nlm.nih.gov/pubmed/29292253 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3969/j.issn.1673-4254.2017.12.08 |
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