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gsdmd基因敲除巨噬细胞RAW 264.7的构建：基于CRISPR/Cas9系统
OBJECTIVE: To construct a cell model of gsdmd gene knockout in macrophage RAW 264.7 cells using CRISPR/Cas9 system. METHODS: Four specific single guide RNAs (sgRNAs) targeting gsdmd were designed to construct pGL3-sgRNA recombinant plasmids, which were identified by PCR amplification and sequencing....
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| Pubblicato in: | Nan Fang Yi Ke Da Xue Xue Bao |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
南方医科大学学报编辑部
2021
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7867478/ https://ncbi.nlm.nih.gov/pubmed/33509763 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.12122/j.issn.1673-4254.2021.01.17 |
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