Purification of Polyglutamine Aggregates and Identification of Elongation Factor-1α and Heat Shock Protein 84 as Aggregate-Interacting Proteins

Aggregates of green fluorescent protein (GFP)-fused truncated N-terminal huntingtin containing abnormally long polyglutamine tracts (150 repeats of glutamine residue) were purified from an ecdysone-inducible mutant neuro2A cell line (HD150Q-28) by using a fluorescence-activated cell sorter. To analy...

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Udgivet i:J Neurosci
Main Authors: Mitsui, Kenichi, Nakayama, Hiroshi, Akagi, Takumi, Nekooki, Munenori, Ohtawa, Kenji, Takio, Koji, Hashikawa, Tsutomu, Nukina, Nobuyuki
Format: Artigo
Sprog:Inglês
Udgivet: Society for Neuroscience 2002
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ARTICLE
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6758042/
https://ncbi.nlm.nih.gov/pubmed/12417652
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1523/JNEUROSCI.22-21-09267.2002
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