Simple 3D culture of dissociated kidney mesenchyme mimics nephron progenitor niche and facilitates nephrogenesis Wnt-independently

Kidney mesenchyme (KM) and nephron progenitors (NPs) depend on WNT activity, and their culture in vitro requires extensive repertoire of recombinant proteins and chemicals. Here we established a robust, simple culture of mouse KM using a combination of 3D Matrigel and growth media supplemented with...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Dapkunas, Arvydas, Rantanen, Ville, Gui, Yujuan, Lalowski, Maciej, Sainio, Kirsi, Kuure, Satu, Sariola, Hannu
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2019
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6748995/
https://ncbi.nlm.nih.gov/pubmed/31530822
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-49526-x
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