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Role of the Carboxylate in Enzyme-Catalyzed Decarboxylation of Orotidine 5′-Monophosphate: Transition State Stabilization Dominates Over Ground State Destabilization

[Image: see text] Kinetic parameters k(ex) (s(–1)) and k(ex)/K(d) (M(–1) s(–1)) are reported for exchange for deuterium in D(2)O of the C-6 hydrogen of 5-fluororotidine 5′-monophosphate (FUMP) catalyzed by the Q215A, Y217F, and Q215A/Y217F variants of yeast orotidine 5′-monophosphate decarboxylase (...

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Bibliografske podrobnosti
izdano v:J Am Chem Soc
Main Authors: Goryanova, Bogdana, Amyes, Tina L., Richard, John P.
Format: Artigo
Jezik:Inglês
Izdano: American Chemical Society 2019
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6735427/
https://ncbi.nlm.nih.gov/pubmed/31365243
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.9b04823
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