Single-Input Regulatory Cascade for in vivo Removal of the Solubility Tag in Fusion Recombinant Proteins Produced by Escherichia coli

Solubility tags are commonly fused to target recombinant proteins to enhance their solubility and stability. In general, these protein tags must be removed to avoid misfolding of the partner protein and to allow for downstream applications. Nevertheless, in vitro tag removal increases process comple...

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Vydáno v:Front Bioeng Biotechnol
Hlavní autoři: Silva, Filipe S. R., Santos, Sara P. O., Meyer, Roberto, Alcantara-Neves, Neuza M., Pinheiro, Carina S., Pacheco, Luis G. C.
Médium: Artigo
Jazyk:Inglês
Vydáno: Frontiers Media S.A. 2019
Témata:
Bioengineering and Biotechnology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6710347/
https://ncbi.nlm.nih.gov/pubmed/31482090
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2019.00200
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