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Recombinant chymosin used for exact and complete removal of a prochymosin derived fusion tag releasing intact native target protein

Fusion tags add desirable properties to recombinant proteins, but they are not necessarily acceptable in the final products. Ideally, fusion tags should be removed releasing the intact native protein with no trace of the tag. Unique endoproteinases with the ability to cleave outside their own recogn...

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Detalhes bibliográficos
Main Authors: Justesen, Sune F L, Lamberth, Kasper, Nielsen, Lise-Lotte B, Schafer-Nielsen, Claus, Buus, Søren
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2771304/
https://ncbi.nlm.nih.gov/pubmed/19388053
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.112
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