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Overexpression of Soluble Recombinant Human Lysyl Oxidase by Using Solubility Tags: Effects on Activity and Solubility

Lysyl oxidase is an important extracellular matrix enzyme that has not been fully characterized due to its low solubility. In order to circumvent the low solubility of this enzyme, three solubility tags (Nus-A, Thioredoxin (Trx), and Glutathione-S-Transferase (GST)) were engineered on the N-terminus...

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Detalhes bibliográficos
Publicado no:Enzyme Res
Main Authors: Smith, Madison A., Gonzalez, Jesica, Hussain, Anjum, Oldfield, Rachel N., Johnston, Kathryn A., Lopez, Karlo M.
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi Publishing Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4753049/
https://ncbi.nlm.nih.gov/pubmed/26942005
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2016/5098985
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