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Preloading budding yeast with all-in-one CRISPR/Cas9 vectors for easy and high-efficient genome editing

The CRISPR/Cas9 technology has greatly improved genome editing in Saccharomyces cerevisiae over recent years. However, several current CRISPR/Cas9 systems suffer from work-intensive cloning procedures and/or the requirement of co-transforming target cells with multiple system components simultaneous...

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Detalhes bibliográficos
Publicado no:J Biol Methods
Main Authors: Degreif, Daniel, Kremenovic, Milana, Geiger, Thomas, Bertl, Adam
Formato: Artigo
Idioma:Inglês
Publicado em: Journal of Biological Methods 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6706142/
https://ncbi.nlm.nih.gov/pubmed/31453248
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14440/jbm.2018.254
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