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Genomic Edition of Ashbya gossypii Using One-vector CRISPR/Cas9

The CRISPR/Cas9 system is a novel genetic tool which allows the precise manipulation of virtually any genomic sequence. In this protocol, we use a specific CRISPR/Cas9 system for the manipulation of Ashbya gossypii. The filamentous fungus A. gossypii is currently used for the industrial production o...

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Bibliografiske detaljer
Udgivet i:Bio Protoc
Main Authors: Muñoz-Fernández, Gloria, Jiménez, Alberto, Revuelta, José Luis
Format: Artigo
Sprog:Inglês
Udgivet: Bio-Protocol 2020
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842619/
https://ncbi.nlm.nih.gov/pubmed/33659330
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3660
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