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Genomic Edition of Ashbya gossypii Using One-vector CRISPR/Cas9
The CRISPR/Cas9 system is a novel genetic tool which allows the precise manipulation of virtually any genomic sequence. In this protocol, we use a specific CRISPR/Cas9 system for the manipulation of Ashbya gossypii. The filamentous fungus A. gossypii is currently used for the industrial production o...
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| Published in: | Bio Protoc |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Bio-Protocol
2020
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7842619/ https://ncbi.nlm.nih.gov/pubmed/33659330 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3660 |
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