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Genomic Edition of Ashbya gossypii Using One-vector CRISPR/Cas9

The CRISPR/Cas9 system is a novel genetic tool which allows the precise manipulation of virtually any genomic sequence. In this protocol, we use a specific CRISPR/Cas9 system for the manipulation of Ashbya gossypii. The filamentous fungus A. gossypii is currently used for the industrial production o...

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Detalles Bibliográficos
Publicado en:Bio Protoc
Main Authors: Muñoz-Fernández, Gloria, Jiménez, Alberto, Revuelta, José Luis
Formato: Artigo
Idioma:Inglês
Publicado: Bio-Protocol 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842619/
https://ncbi.nlm.nih.gov/pubmed/33659330
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3660
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