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Targeted transcript quantification in single disseminated cancer cells after whole transcriptome amplification

Gene expression analysis of rare or heterogeneous cell populations such as disseminated cancer cells (DCCs) requires a sensitive method allowing reliable analysis of single cells. Therefore, we developed and explored the feasibility of a quantitative PCR (qPCR) assay to analyze single-cell cDNA pre-...

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Veröffentlicht in:PLoS One
Hauptverfasser: Durst, Franziska C., Grujovic, Ana, Ganser, Iris, Hoffmann, Martin, Ugocsai, Peter, Klein, Christoph A., Czyż, Zbigniew T.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2019
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6701776/
https://ncbi.nlm.nih.gov/pubmed/31430289
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0216442
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