Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization

Rare target cells can be isolated from a high background of non-target cells using antibodies specific for surface proteins of target cells. A recently developed method uses a medical wire functionalized with anti-epithelial cell adhesion molecule (EpCAM) antibodies for in vivo isolation of circulat...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Chen, Shukun, El-Heliebi, Amin, Schmid, Julia, Kashofer, Karl, Czyż, Zbigniew T., Polzer, Bernhard Michael, Pantel, Klaus, Kroneis, Thomas, Sedlmayr, Peter
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2018
Fag:
Cancer Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101176/
https://ncbi.nlm.nih.gov/pubmed/29863657
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56394
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