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Transcript amplification from single bacterium for transcriptome analysis

Total transcript amplification (TTA) from single eukaryotic cells for transcriptome analysis is established, but TTA from a single prokaryotic cell presents additional challenges with much less starting material, the lack of poly(A)-tails, and the fact that the messages can be polycistronic. Here, w...

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Hlavní autoři: Kang, Yun, Norris, Michael H., Zarzycki-Siek, Jan, Nierman, William C., Donachie, Stuart P., Hoang, Tung T.
Médium: Artigo
Jazyk:Inglês
Vydáno: Cold Spring Harbor Laboratory Press 2011
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3106325/
https://ncbi.nlm.nih.gov/pubmed/21536723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.116103.110
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