Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

BACKGROUND: The microbial transglutaminase (MTG) is inactive when only the mature sequence is expressed in Pichia pastoris. Although co-expression of MTG and its N-terminal pro-peptide can obtain the active MTG, the enzyme activity was still low. One of the basic steps for strain improvement is to e...

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Vydáno v:BMC Biotechnol
Hlavní autoři: Song, Xiaoping, Shao, Changsheng, Guo, Yugang, Wang, Yajie, Cai, Jingjing
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2019
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6668168/
https://ncbi.nlm.nih.gov/pubmed/31362722
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-019-0542-6
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