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Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris
BACKGROUND: The microbial transglutaminase (MTG) is inactive when only the mature sequence is expressed in Pichia pastoris. Although co-expression of MTG and its N-terminal pro-peptide can obtain the active MTG, the enzyme activity was still low. One of the basic steps for strain improvement is to e...
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| Publicado no: | BMC Biotechnol |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6668168/ https://ncbi.nlm.nih.gov/pubmed/31362722 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-019-0542-6 |
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