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An acyl-CoA dehydrogenase microplate activity assay using recombinant porcine electron transfer flavoprotein

Acyl-CoA dehydrogenases (ACADs) play key roles in the mitochondrial catabolism of fatty acids and branched-chain amino acids. All nine characterized ACAD enzymes use electron transfer flavoprotein (ETF) as their redox partner. The gold standard for measuring ACAD activity is the anaerobic ETF fluore...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Anal Biochem
Hauptverfasser: Zhang, Yuxun, Mohsen, Al-Walid, Kochersperger, Catherine, Solo, Keaton, Schmidt, Alexandra V., Vockley, Jerry, Goetzman, Eric S.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2019
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6661201/
https://ncbi.nlm.nih.gov/pubmed/31194945
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2019.06.003
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