Troubleshooting fine‐tuning procedures for qPCR system design

Quantitative real‐time PCR (qPCR) has been improved and optimized over the past decade for a wide range of applications. Design of primers and probes is one of the crucial steps to obtain high system efficiency of qPCR since design pitfalls influence negatively amplification performances. We report...

Full description

Saved in:
Bibliographic Details
Published in:J Clin Lab Anal
Main Authors: Raso, Alessandro, Mascelli, Samantha, Nozza, Paolo, Ugolotti, Elisabetta, Vanni, Irene, Capra, Valeria, Biassoni, Roberto
Format: Artigo
Language:Inglês
Published: Wiley Subscription Services, Inc., A Wiley Company 2011
Subjects:
Original Articles
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6647679/
https://ncbi.nlm.nih.gov/pubmed/22086791
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jcla.20489
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items