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Validation of kinetics similarity in qPCR

Quantitative real-time PCR (qPCR) is the method of choice for specific and sensitive quantification of nucleic acids. However, data validation is still a major issue, partially due to the complex effect of PCR inhibition on the results. If undetected PCR inhibition may severely impair the accuracy a...

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Bibliographic Details
Main Authors: Bar, Tzachi, Kubista, Mikael, Tichopad, Ales
Format: Artigo
Language:Inglês
Published: Oxford University Press 2012
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3287174/
https://ncbi.nlm.nih.gov/pubmed/22013160
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr778
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