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Correction of RT–qPCR data for genomic DNA-derived signals with ValidPrime
Genomic DNA (gDNA) contamination is an inherent problem during RNA purification that can lead to non-specific amplification and aberrant results in reverse transcription quantitative PCR (RT—qPCR). Currently, there is no alternative to RT(−) controls to evaluate the impact of the gDNA background on...
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| Autori principali: | , , , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
2012
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3326333/ https://ncbi.nlm.nih.gov/pubmed/22228834 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkr1259 |
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