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How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments

We have examined the imprecision in the estimation of PCR efficiency by means of standard curves based on strategic experimental design with large number of technical replicates. In particular, how robust this estimation is in terms of a commonly varying factors: the instrument used, the number of t...

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Bibliografski detalji
Izdano u:Biomol Detect Quantif
Glavni autori: Svec, David, Tichopad, Ales, Novosadova, Vendula, Pfaffl, Michael W., Kubista, Mikael
Format: Artigo
Jezik:Inglês
Izdano: Elsevier 2015
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4822216/
https://ncbi.nlm.nih.gov/pubmed/27077029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bdq.2015.01.005
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