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How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments

We have examined the imprecision in the estimation of PCR efficiency by means of standard curves based on strategic experimental design with large number of technical replicates. In particular, how robust this estimation is in terms of a commonly varying factors: the instrument used, the number of t...

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Detalhes bibliográficos
Publicado no:Biomol Detect Quantif
Main Authors: Svec, David, Tichopad, Ales, Novosadova, Vendula, Pfaffl, Michael W., Kubista, Mikael
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4822216/
https://ncbi.nlm.nih.gov/pubmed/27077029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bdq.2015.01.005
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