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How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments

We have examined the imprecision in the estimation of PCR efficiency by means of standard curves based on strategic experimental design with large number of technical replicates. In particular, how robust this estimation is in terms of a commonly varying factors: the instrument used, the number of t...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Biomol Detect Quantif
Egile Nagusiak: Svec, David, Tichopad, Ales, Novosadova, Vendula, Pfaffl, Michael W., Kubista, Mikael
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Elsevier 2015
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4822216/
https://ncbi.nlm.nih.gov/pubmed/27077029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bdq.2015.01.005
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