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The kinetic requirements of extreme qPCR

The kinetic requirements of quantitative PCR were experimentally dissected into the stages of DNA denaturation, primer annealing, and polymerase extension. The temperature/time conditions for 2 stages were kept optimal, while the other was limited until the amplification efficiency decreased as meas...

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Detalhes bibliográficos
Publicado no:Biomol Detect Quantif
Main Authors: Millington, Adam L., Houskeeper, Jessica A., Quackenbush, John F., Trauba, James M., Wittwer, Carl T.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6591793/
https://ncbi.nlm.nih.gov/pubmed/31285997
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bdq.2019.100081
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