The kinetic requirements of extreme qPCR

The kinetic requirements of quantitative PCR were experimentally dissected into the stages of DNA denaturation, primer annealing, and polymerase extension. The temperature/time conditions for 2 stages were kept optimal, while the other was limited until the amplification efficiency decreased as meas...

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Vydáno v:Biomol Detect Quantif
Hlavní autoři: Millington, Adam L., Houskeeper, Jessica A., Quackenbush, John F., Trauba, James M., Wittwer, Carl T.
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2019
Témata:
Special Issue on Liquid Biopsy & Biomarkers, Edited by Dr Michael Pfaffl
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6591793/
https://ncbi.nlm.nih.gov/pubmed/31285997
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bdq.2019.100081
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