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Troubleshooting fine‐tuning procedures for qPCR system design

Quantitative real‐time PCR (qPCR) has been improved and optimized over the past decade for a wide range of applications. Design of primers and probes is one of the crucial steps to obtain high system efficiency of qPCR since design pitfalls influence negatively amplification performances. We report...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:J Clin Lab Anal
Main Authors: Raso, Alessandro, Mascelli, Samantha, Nozza, Paolo, Ugolotti, Elisabetta, Vanni, Irene, Capra, Valeria, Biassoni, Roberto
Format: Artigo
Jezik:Inglês
Izdano: Wiley Subscription Services, Inc., A Wiley Company 2011
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6647679/
https://ncbi.nlm.nih.gov/pubmed/22086791
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jcla.20489
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