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Troubleshooting fine‐tuning procedures for qPCR system design

Quantitative real‐time PCR (qPCR) has been improved and optimized over the past decade for a wide range of applications. Design of primers and probes is one of the crucial steps to obtain high system efficiency of qPCR since design pitfalls influence negatively amplification performances. We report...

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Vydáno v:J Clin Lab Anal
Hlavní autoři: Raso, Alessandro, Mascelli, Samantha, Nozza, Paolo, Ugolotti, Elisabetta, Vanni, Irene, Capra, Valeria, Biassoni, Roberto
Médium: Artigo
Jazyk:Inglês
Vydáno: Wiley Subscription Services, Inc., A Wiley Company 2011
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6647679/
https://ncbi.nlm.nih.gov/pubmed/22086791
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jcla.20489
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