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Luminescent peptide tagging enables efficient screening for CRISPR-mediated knock-in in human induced pluripotent stem cells
Human pluripotent stem cells are increasingly used for CRISPR-mediated gene targeting in efforts to generate models of human diseases. This is a challenging task because of the high sensitivity of these cells to suboptimal conditions, including CRISPR-associated DNA damage and subsequent rounds of s...
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| Pubblicato in: | Wellcome Open Res |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
F1000 Research Limited
2019
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6640264/ https://ncbi.nlm.nih.gov/pubmed/31363496 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.12688/wellcomeopenres.15119.3 |
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