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CRISPR/Cas9 Mediated Fluorescent Tagging of Endogenous Proteins in Human Pluripotent Stem Cells

Human induced pluripotent stem cells (hiPSCs) can be used to mass-produce surrogates of human tissues, enabling new advances in drug screening, disease modeling, and cell therapy. Recent developments in CRISPR/Cas9 genome editing technology use homology directed repair (HDR) to efficiently generate...

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Détails bibliographiques
Publié dans:Curr Protoc Hum Genet
Auteurs principaux: Sharma, Arun, Toepfer, Christopher N., Ward, Tarsha, Wasson, Lauren, Agarwal, Radhika, Conner, David A., Hu, Johnny H., Seidman, Christine E.
Format: Artigo
Langue:Inglês
Publié: 2018
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5785097/
https://ncbi.nlm.nih.gov/pubmed/29364519
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cphg.52
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