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CRISPR/Cas9 Mediated Fluorescent Tagging of Endogenous Proteins in Human Pluripotent Stem Cells
Human induced pluripotent stem cells (hiPSCs) can be used to mass-produce surrogates of human tissues, enabling new advances in drug screening, disease modeling, and cell therapy. Recent developments in CRISPR/Cas9 genome editing technology use homology directed repair (HDR) to efficiently generate...
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| Publié dans: | Curr Protoc Hum Genet |
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| Auteurs principaux: | , , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5785097/ https://ncbi.nlm.nih.gov/pubmed/29364519 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cphg.52 |
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