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A piggyBac-based toolkit for inducible genome editing in mammalian cells
We describe the development and application of a novel series of vectors that facilitate CRISPR-Cas9-mediated genome editing in mammalian cells, which we call CRISPR-Bac. CRISPR-Bac leverages the piggyBac transposon to randomly insert CRISPR-Cas9 components into mammalian genomes. In CRISPR-Bac, a s...
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| Publicado no: | RNA |
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| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6633203/ https://ncbi.nlm.nih.gov/pubmed/31101683 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.068932.118 |
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