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A piggyBac-based toolkit for inducible genome editing in mammalian cells

We describe the development and application of a novel series of vectors that facilitate CRISPR-Cas9-mediated genome editing in mammalian cells, which we call CRISPR-Bac. CRISPR-Bac leverages the piggyBac transposon to randomly insert CRISPR-Cas9 components into mammalian genomes. In CRISPR-Bac, a s...

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Bibliografski detalji
Izdano u:RNA
Glavni autori: Schertzer, Megan D., Thulson, Eliza, Braceros, Keean C.A., Lee, David M., Hinkle, Emma R., Murphy, Ryan M., Kim, Susan O., Vitucci, Eva C.M., Calabrese, J. Mauro
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2019
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6633203/
https://ncbi.nlm.nih.gov/pubmed/31101683
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.068932.118
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