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Quantitative mapping of fluorescently tagged cellular proteins using FCS-calibrated four dimensional imaging
The ability to tag a protein at its endogenous locus with a fluorescent protein (FP) enables the quantitative understanding of protein dynamics at the physiological level. Genome editing technology has now made this powerful approach routinely applicable to mammalian cells and many other model syste...
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| Pubblicato in: | Nat Protoc |
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| Autori principali: | , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2018
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6609853/ https://ncbi.nlm.nih.gov/pubmed/29844523 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2018.040 |
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