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Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells

Fluorescence tagging of proteins is a widely used tool to study protein function and dynamics in live cells. However, the extent to which different mammalian transgene methods faithfully report on the properties of endogenous proteins has not been studied comparatively. Here we use quantitative live...

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Dettagli Bibliografici
Pubblicato in:Mol Biol Cell
Autori principali: Mahen, Robert, Koch, Birgit, Wachsmuth, Malte, Politi, Antonio Z., Perez-Gonzalez, Alexis, Mergenthaler, Julia, Cai, Yin, Ellenberg, Jan
Natura: Artigo
Lingua:Inglês
Pubblicazione: The American Society for Cell Biology 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4230620/
https://ncbi.nlm.nih.gov/pubmed/25232003
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E14-06-1091
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