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Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells

Fluorescence tagging of proteins is a widely used tool to study protein function and dynamics in live cells. However, the extent to which different mammalian transgene methods faithfully report on the properties of endogenous proteins has not been studied comparatively. Here we use quantitative live...

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Detalhes bibliográficos
Publicado no:Mol Biol Cell
Main Authors: Mahen, Robert, Koch, Birgit, Wachsmuth, Malte, Politi, Antonio Z., Perez-Gonzalez, Alexis, Mergenthaler, Julia, Cai, Yin, Ellenberg, Jan
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Cell Biology 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4230620/
https://ncbi.nlm.nih.gov/pubmed/25232003
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E14-06-1091
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