Quantitative mapping of fluorescently tagged cellular proteins using FCS-calibrated four dimensional imaging

The ability to tag a protein at its endogenous locus with a fluorescent protein (FP) enables the quantitative understanding of protein dynamics at the physiological level. Genome editing technology has now made this powerful approach routinely applicable to mammalian cells and many other model syste...

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Kaydedildi:
Detaylı Bibliyografya
Yayımlandı:Nat Protoc
Asıl Yazarlar: Politi, Antonio Z., Cai, Yin, Walther, Nike, Hossain, M. Julius, Koch, Birgit, Wachsmuth, Malte, Ellenberg, Jan
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2018
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC6609853/
https://ncbi.nlm.nih.gov/pubmed/29844523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2018.040
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