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Quantitative mapping of fluorescently tagged cellular proteins using FCS-calibrated four dimensional imaging

The ability to tag a protein at its endogenous locus with a fluorescent protein (FP) enables the quantitative understanding of protein dynamics at the physiological level. Genome editing technology has now made this powerful approach routinely applicable to mammalian cells and many other model syste...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Politi, Antonio Z., Cai, Yin, Walther, Nike, Hossain, M. Julius, Koch, Birgit, Wachsmuth, Malte, Ellenberg, Jan
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6609853/
https://ncbi.nlm.nih.gov/pubmed/29844523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2018.040
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