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Generation and validation of homozygous fluorescent knock-in cells using CRISPR/Cas9 genome editing
Gene tagging with fluorescent proteins is essential to investigate the dynamic properties of cellular proteins. CRISPR/Cas9 technology is a powerful tool for inserting fluorescent markers into all alleles of the gene of interest (GOI) and permits functionality and physiological expression of the fus...
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| Publicado en: | Nat Protoc |
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| Autores principales: | , , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6556379/ https://ncbi.nlm.nih.gov/pubmed/29844520 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2018.042 |
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