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The Acidaminococcus sp. Cas12a nuclease recognizes GTTV and GCTV as non-canonical PAMs

The clustered regularly interspaced short palindromic repeat (CRISPR)-associated (Cas) nuclease Acidaminococcus sp. Cas12a (AsCas12a, also known as AsCpf1) has become a popular alternative to Cas9 for genome editing and other applications. AsCas12a has been associated with a TTTV protospacer-adjacen...

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Bibliografiske detaljer
Udgivet i:FEMS Microbiol Lett
Main Authors: Jacobsen, Thomas, Liao, Chunyu, Beisel, Chase L
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6604746/
https://ncbi.nlm.nih.gov/pubmed/31004485
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/femsle/fnz085
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