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BioID as a Tool for Protein-Proximity Labeling in Living Cells

BioID has become an increasingly utilized tool for identifying candidate protein-protein interactions (PPIs) in living cells. This method utilizes a promiscuous biotin ligase, called BioID, fused to a protein-of-interest that when expressed in cells can be induced to biotinylate interacting and prox...

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Bibliographic Details
Published in:Methods Mol Biol
Main Authors: Sears, Rhiannon M., May, Danielle G., Roux, Kyle J.
Format: Artigo
Language:Inglês
Published: 2019
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6583792/
https://ncbi.nlm.nih.gov/pubmed/31161514
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-9546-2_15
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