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BioID as a Tool for Protein-Proximity Labeling in Living Cells
BioID has become an increasingly utilized tool for identifying candidate protein-protein interactions (PPIs) in living cells. This method utilizes a promiscuous biotin ligase, called BioID, fused to a protein-of-interest that when expressed in cells can be induced to biotinylate interacting and prox...
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| Publié dans: | Methods Mol Biol |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2019
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6583792/ https://ncbi.nlm.nih.gov/pubmed/31161514 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-9546-2_15 |
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