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An improved method for isolation of mitochondria from cell lines that enables reconstitution of calcium-dependent processes
Optimum cytosolic calcium concentrations support balanced mitochondrial respiration. However, the concentration of cytosolic Ca(2+) varies among cell types and excess cytosolic Ca(2+) can cause mitochondrial dysfunction, particularly when the mitochondria are isolated from cell lines. We optimized a...
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| 出版年: | Anal Biochem |
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| 主要な著者: | , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2019
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6582631/ https://ncbi.nlm.nih.gov/pubmed/31022379 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2019.04.012 |
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