Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing

CRISPR‐Cas9 and Cas12a are two powerful genome editing systems. Expression of CRISPR in plants is typically achieved with a mixed dual promoter system, in which Cas protein is expressed by a Pol II promoter and a guide RNA is expressed by a species‐specific Pol III promoter such as U6 or U3. To achi...

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Bibliographische Detailangaben
Veröffentlicht in:Plant Biotechnol J
Hauptverfasser: Tang, Xu, Ren, Qiurong, Yang, Lijia, Bao, Yu, Zhong, Zhaohui, He, Yao, Liu, Shishi, Qi, Caiyan, Liu, Binglin, Wang, Yan, Sretenovic, Simon, Zhang, Yingxiao, Zheng, Xuelian, Zhang, Tao, Qi, Yiping, Zhang, Yong
Format: Artigo
Sprache:Inglês
Veröffentlicht: John Wiley and Sons Inc. 2019
Schlagworte:
Research Articles
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6576101/
https://ncbi.nlm.nih.gov/pubmed/30582653
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/pbi.13068
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