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Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing
CRISPR‐Cas9 and Cas12a are two powerful genome editing systems. Expression of CRISPR in plants is typically achieved with a mixed dual promoter system, in which Cas protein is expressed by a Pol II promoter and a guide RNA is expressed by a species‐specific Pol III promoter such as U6 or U3. To achi...
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| 出版年: | Plant Biotechnol J |
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| 主要な著者: | , , , , , , , , , , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
John Wiley and Sons Inc.
2019
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6576101/ https://ncbi.nlm.nih.gov/pubmed/30582653 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/pbi.13068 |
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