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Peptide:MHCII tetramer-based cell enrichment for the study of epitope-specific CD4(+) T cells.

Epitope-specific CD4(+) T cells can be labeled in complex cell mixtures from secondary lymphoid organs with fluorophore-labeled p:MHCII tetramers and then detected by flow cytometry. Magnetic enrichment of tetramer-bound cells before flow cytometry increases the sensitivity of detection to the point...

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Bibliografski detalji
Izdano u:Curr Protoc Immunol
Glavni autori: Kotov, Dmitri I., Jenkins, Marc K.
Format: Artigo
Jezik:Inglês
Izdano: 2019
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6570544/
https://ncbi.nlm.nih.gov/pubmed/31034767
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpim.75
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