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Peptide:MHCII tetramer-based cell enrichment for the study of epitope-specific CD4(+) T cells.
Epitope-specific CD4(+) T cells can be labeled in complex cell mixtures from secondary lymphoid organs with fluorophore-labeled p:MHCII tetramers and then detected by flow cytometry. Magnetic enrichment of tetramer-bound cells before flow cytometry increases the sensitivity of detection to the point...
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| Publicado no: | Curr Protoc Immunol |
|---|---|
| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6570544/ https://ncbi.nlm.nih.gov/pubmed/31034767 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpim.75 |
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