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Design and synthesis of fluorescent activity probes for protein phosphatases

Protein phosphatases act in concert with protein kinases to regulate and maintain the phosphoproteome. However, the catalog of chemical tools to directly monitor the enzymatic activity of phosphatases has lagged behind their kinase counterparts. In this chapter, we provide protocols for repurposing...

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Bibliographic Details
Published in:Methods Enzymol
Main Authors: Casey, Garrett R., Beck, Jon R., Stains, Cliff l.
Format: Artigo
Language:Inglês
Published: 2019
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6567983/
https://ncbi.nlm.nih.gov/pubmed/31155057
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2019.02.002
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