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Quantification of Protein Kinase Enzymatic Activity in Unfractionated Cell Lysates Using CSox-Based Sensors
Defining perturbations in protein kinase activity within biological samples can provide insight into disease mechanisms as well as potential targets for drug development. In this protocol, we present a method that utilizes a phosphorylation-sensitive amino acid, termed CSox, to afford kinase-selecti...
में बचाया:
| मुख्य लेखकों: | , , , |
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| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
2014
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4174361/ https://ncbi.nlm.nih.gov/pubmed/25205563 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/9780470559277.ch140106 |
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