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The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping

Eukaryotic mRNAs are modified at their 5′ end early during transcription by the addition of N7-methylguanosine (m(7)G), which forms the “cap” on the first 5′ nucleotide. Identification of the 5′ nucleotide on mRNA is necessary for determination of the Transcription Start Site (TSS). We explored the...

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Библиографические подробности
Опубликовано в: :Sci Rep
Главные авторы: Wulf, Madalee G., Buswell, John, Chan, Siu-Hong, Dai, Nan, Marks, Katherine, Martin, Evan R., Tzertzinis, George, Whipple, Joseph M., Corrêa, Ivan R., Schildkraut, Ira
Формат: Artigo
Язык:Inglês
Опубликовано: Nature Publishing Group UK 2019
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC6565619/
https://ncbi.nlm.nih.gov/pubmed/31197197
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-45083-5
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